NAME ___________________________________ CLASS _____________________________

Chapter 10 Bone Tissue and Bones go to the following topics/virtual histology slides on the University of Michigan web site http://www.med.umich.edu/histology/dmindex.html

 


Lab Activity 2 - Bone Ground Page 210

University of Michigan support for this Lab Activity.

Ground section of compact bone, cross, 40X. This slide is similar to Fig. 10.14 in workbook.

Identify the following structures on this slide: osteon=Haversian system, osteocytes, Haversian (central) canal, canaliculi, matrix, lamellae).

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Ground section of compact bone, cross, 40X This slide is similar to Fig. 10.14 in workbook.

Identify the following structures on this slide: osteon=Haversian system,

osteocytes, Haversian (central) canal, canaliculi, matrix, lamellae).

WebScope

 

 

Ground section of compact bone, longitudinal section, 40X (thin section).

Identify the following structures on this slide: osteon=Haversian system,

osteocytes, Haversian (central) canal, canaliculi, matrix, lamellae).

WebScope

Lab Activity 3 - Bone Demineralized Page 211

University of Michigan support for this Lab Activity. This slide is a cross section of a long bone with surrounding cross-sectioned skeletal muscle with tendon. This slide shows several sites of Periosteum to Tendon Junctions (see Workbook Lab Activity 12, slide is similar to Figure 10.16, page 221 and in the Worksheets page 115, Fig. 8.24).

Decalcified bone, cross section, H&E, 20X

Identify the following structures on this slide: osteon=Haversian system,

osteocytes, Haversian (central) canal, collagen fibers, lamellae, periosteum,

endosteum, bone marrow, blood vessels in bone marrow).

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Lab Activity 4 Intramembranous Ossification Page 214

University of Michigan support for this Lab Activity. Observe the palate for structure of intramembranous ossification.

Palate, saggital section, H&E, 40X. This slide is similar to Figures 10.23 and 10.24 of workbook.

Identify region of palate showing intramembranous bone formation. Identify

fibrous membrane, region of spongy bone (look for trabeculae), region of

compact bone (look for a group of osteons in cross-section), periosteum,

endosteum, osteocytes, osteoblasts, newly forming osteons, blood vessels,

collagen fibers of the bone.

 

 

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 Additionally, the following is a slide of intramembranous ossification of the maxilla (a flat bone but with irregular shape). Notice the developing trabeculae in the maxilla.

Fetal face and jaw, frontal section, H&E, 40X

Identify region showing intramembranous bone formation. Identify

fibrous membrane, region of spongy bone (look for trabeculae),

periosteum, osteocytes, osteoblasts, blood vessels, collagen fibers of bone. Is

this slide development younger or older than the above slide preparation?

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Lab Activity 7 Endochondrial Ossification Page 216

University of Michigan support for this Lab Activity. This slide is a longitudinal section of a long bone (finger) with the middle phalanx similar to Fig. 10.34 of workbook).

Finger, H&E, 20X

Why is this an example of endochondral bone formation? Identify proximal and distal epiphyses, proximal and distal metaphyses, diaphysis, hyaline cartilage (region of articulation, region of proliferation, region of hypertrophy, and region of cavitations), trabeculae, fibrous and cellular layers of periosteum, osteons (forming and developed), endosteum, osteoblasts, osteocytes, compact bone, medullary cavity (forming), bone marrow, dense connective tissue of joint.

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Lab Activity 9 Endochondrial Ossification Page 219

University of Michigan support for this Lab Activity. This slide is a longitudinal section of a long bone similar to Fig. 10.40 of workbook).

Developing long bone, H&E, 20X

Identify epiphyses, metaphyses, diaphysis, hyaline cartilage (region of articulation, region of proliferation, region of hypertrophy, and region of cavitations), trabeculae, fibrous and cellular layers of periosteum, osteons (forming and developed), endosteum, osteoblasts, osteocytes, compact bone, medullary cavity (forming), bone marrow. Does this slide show a secondary ossification center? Does this slide show appositional growth?

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Lab Activity 12 Decalcified Bone showing Appositional Growth Page 221

University of Michigan support for this Lab Activity.

Fetal leg, cross section, H&E, 40X. This slide is similar to Figure 10.46 and 10.47 of workbook.)

WebScope

Identify spongy bone, trabeculae, fibrous and cellular layers of periosteum, tendon to periosteum junctions, osteons (forming and developed), endosteum, osteoblasts, osteocytes, compact bone, medullary cavity (forming), bone marrow. Does this slide show longitudinal growth?

 

Decalcified bone, cross section, H&E, 20X (compact bone, osteon=Haversian system). This slide is similar to Figure 10.46 and 10.47 of workbook.)

WebScope

Identify compact bone, fibrous and cellular layers of periosteum, tendon to periosteum junctions, osteons (forming and developed), endosteum, osteoblasts, osteocytes, medullary cavity, bone marrow. Does this slide show longitudinal growth? Is the bone development of this slide older or younger than the prior (above) slide?

Lab Activity 13 Thyroid and Parathyroid Glands Page 222

University of Michigan support for this Lab Activity. This slide is mostly thyroid gland. The parathyroid is on small area at the bottom right as you view the slide.
This slide is similar to Figure 10.50 of workbook.

Thyroid gland and parathyroid, H&E, 40X

Identify thyroid, follicles, follicular epithelial cells, colloid, parathyroid (at lower right). Know stimulus, function, and target of hormones.

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Lab Activity 14 Ground Bone showing Remodeling Page 224

University of Michigan support for this Lab Activity. This slide is the same that was presented in Lab Activity 2, page 210.
Observe the slide for remodeling which is similar to Figure 10.53 of workbook.

Ground section of compact bone, cross, 40X

Identify osteon=Haversian system, osteocytes, canaliculi, interstitial lamellae. Why are central canals on left side (as you view the image) a different size than those on the right side?

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